Representative methylation analysis of DL samples from carriers in whom aberrant methylation was found
Imogen Locke, Kote-Jarai Zsofia, Fackler, Mary Jo, Bancroft Elizabeth, Peter Osin, Ashutosh Y. Nerurkar, Izatt Louise, Gabriella Pichert, Gui, Gerald PH, Eeles, Rosalind A · Figshare · 2011
Copyright information:Taken from "Gene promoter hypermethylation in ductal lavage fluid from healthy gene mutation carriers and mutation-negative controls"Breast Cancer Research 2007;9(1):R20-R20.Published online 26 Feb 2007PMCID:PMC1851387. Hypermethylation of found in ductal lavage (DL) samples from subjects 17, 11, 3 and 6. Replicate PCR experiments for subject 11, using DNA from the same duct, are shown to illustrate reproducibility. Methylation analysis of . Two different ducts for subject 1 are shown: the first is unmethylated (duct right 2a; see Figure 2) and the second shows hypermethylation of (duct right 1a). Subjects 11, 13 and 8 show aberrant methylation of HIN-1. In both panels, unmethylated control samples consisted of 1 mg/ml solutions of human sperm DNA (HSD), and methylated controls were 1 mg/ml solutions of DNA extracted from the MDA-MB-231 breast cancer cell line (231). Marker used is a 123bp DNA ladder - rungs represent 123bp, 246bp and 369bp. M, methylated; U, unmethylated.