Identification and validation of micrornas for diagnosing type 2 diabetes : an in silico and molecular approach
Yancke Anthony · University of the Western Cape Electronic Theses and Dissertations Repository (University of the Western Cape) · 2015
Type 2 diabetes mellitus (T2DM), a metabolic disease characterized by chronic hyperglycemia, is the most prevalent form of diabetes globally, affecting approximately 95 % of the total number of people with diabetes i.e. approximately 366 million.Furthermore, it is also the most prevalent form in South Africa (SA), iii Future work, therefore, include validation of the predicted target genes to the miRNAs of interest i.e. miR-dr-1: PPARA and LDLR and miR-ur-1: CACNB2, using molecular approaches such as the luciferase assays and western blots.