Analysis of Nuclei Detection with Stain Normalization in Histopathology Images
Sabeena Beevi K, G. R. Bindu · Indian Journal of Science and Technology · 2015
This paper proposes a unique detection technique to identify nuclei from microscopy images of breast histopathology slides. The method involves stain normalization, color decomposition and hysteresis thresholding. A comparative analysis of different pre-processed images on nuclei detection reveals an accuracy of 97% for Blue Ratio (BR) images. The technique uses three color channels for accurate detection of nuclei. An analysis of two stain normalization procedure is also presented.Keywords: Blue Ratio Image, Histopathology, Stain Normalization