Hematoxylin Basic Fuchsin Picric Acid (HBFP) Method for Morphological Evaluation of Fifty Cases of Myocardial Infarction
Z. Bogović, Hrvoje Čupić · University of Zagreb University Computing Centre (SRCE) · 2005
AIMS: To evaluate the utility and accuracy of intraoperative imprint cytology (IIC) of sentinel lymph nodes (SLNs) in patients with metastatic melanoma, and to establish this diagnostic procedure in Croatia.METHODS: Seventy-two sentinel lymph nodes from patients with metastatic melanoma were identified by technetium-labeled sulfur colloid and blue dye.SLNs were evaluated intraoperatively by slicing into 3-mm sections.Imprints were made of each cut surface, stained with Hemacolor quick method and examined by a cytologist.Permanent cytologic imprints were stained with Pappenheim and immunocytochemical staining for S-100 and HMB-45 proteins.Intraoperative imprint cytology results were compared with final histologic ones.RESULTS: A total of 72 SLNs were excised from 47 patients with malignant melanoma (1.6 SLNs per patient).Metastases were identified in 13 (27.5%)patients.The sensitivity of IIC for SNL was 75% (12/16) per sentinel node and 69% (9/13) per patient.All 4 false-negative SLNs by IIC were detected in patients with isolated cells of melanoma in permanent histopathologic sections.No falsepositive results were identified (100% specificity).CONCLUSIONS: IIC of SNLs of metastatic melanoma is a reliable and accurate intraoperative technique.It could be used as an alternative to frozen section, especially because it is rapid, cost-effective and tissue saving.