INFLUENCE OF NEWLY SYNTHESIZED MIXED LIGAND IRON COMPLEXES ON VIABILITY AND PROLIFERATION OF TUMOR CELLS
Tanya Zhivkova, Reni E. Kalfin, Lora Dyakova, E. Leventieva-Necheva, Ramona Tudose, Otilia Costişor, Radostina Alexandrova · 2010
PURPOSE: The aim of the study presented here was to evaluate the putative cytotoxic and antiproliferative activities of two mixed ligand iron (II, III) complexes containing Mannich base N,N’-bis(4-antipyrylmethyl)-piperazine (BAMP) and pyridine (py, C5H5N) as a co-ligand. MATERIALS AND METHODS: The following permanent cell lines were used as experimental models in our investigations: LSCC-SF-Mc29 (chicken hepatoma); LSR-SF-SR (rat sarcoma) and 8 MGBA (human glioblastoma multiforme). The influence of the compounds on cell viability and proliferation was examined by neutral red uptake cytotoxicity assay and colony-forming method. RESULTS: The results obtained revealed that the examined mixed ligand iron complexes express low cytotoxic and antiproliferative activities when applied at concentrations of 10-200 µg/ml for 24 to 48 h. Tested independently, the ligand BAMP was no or very low toxic at the concentrations examined. CONCLUSION: Based on their sensitivity to the toxic effects of the tested compounds, the cell lines used in our experiments are graded as follows: LSCC-SF(Mc29) > LSR-SF(SR) > 8 MGBA.