Optimized pyrogallol based superoxide scavenging assays for testing antioxidants
A. Mir · 2015
Four in vitro methods have been optimized for detection of superoxide generated from autoxidation of pyrogallol in alkaline solution. The methods have been linear over 0.1 through 1.0 µmole pyrogallol with pyrogallol autooxidation assay (PAA), pyrogallol autoxidation assay in presence of iron (II) (PAAI) and iodometric pyrogallol autoxidation assay (IPAA), and over 0.04 through 0.20 µmole pyrogallol with pyrogallol-mediated MTT reduction assay (PMTTRA).The relative sensitivity order of the assays based on their regression estimates (the mean values indicated within parenthesis) has been: PMTTRA (2.464) >> PAAI (0.596) ≥ PAA (0.568)>IPAA (0.283). Flavonoids, phenolics, thio compounds, ascorbic acid and anionic salts have differently responded to these assays. The assays provide inexpensive tools to employ these as a part of any primary screening program for testing antioxidants for any scavenging activity against superoxide radicals.