Cytotoxic activity and Antioxidant Potentials of hexane and Methanol extracts of IR64 Rice bran against Human Lung (A549) and Colon (HCT116) Carcinomas
Mark Lloyd, Mark Lloyd Granaderos Dapar, Jonathan F. Garzon, Cesar G. Demayo · 2013
Rice bran is a byproduct of rice milling process which contains significant amount of natural phytochemicals. The IR64 rice variety is a high-yielding, semi-dwarf and mostly grown variety in the tropics. This study aims to determine the cytotoxic and antioxidant potentials of IR64 rice bran. The sample was subjected to sequential extraction using hexane and ethanol. The concentrated extracts were diluted in dimethyl sulfoxide (DMSO). The cytotoxic activity of hexane and ethanol extracts was analyzed using the Methyl Thiazol Tetrazollium (MTT) Assay for human lung and colon carcinomas. Both extracts were also analyzed by Cytotoxicity Assay using lymphocytes from normal blood. The antioxidant property was evaluated by free radical diphenyl-picrylhydrazyl (DPPH) scavenging assay. The result of MTT Assay revealed that ethanol extract inhibit proliferation of human lung and colon carcinomas at 10181 µg/ml and 6650 µg/ml, respectively. The ethanol extract demonstrated 79.79% inhibition against DPPH. Based on the cytoxicity assay conducted, it shows that the sample is not toxic to normal cells (lymphocytes) having an average of only 14 cells died at a 193 cell population (7.25 per 100 cells) compared to the base cell medium as untreated control and 2.5% DMSO in phosphate buffered saline (PBS). No cytotoxicity and antioxidant potentials were exhibited by the hexane extract. It was concluded that IR64 rice bran ethanol extract is a potential source of bioactive compound/s against both human lung and colon carcinomas and a potential antioxidant against free radicals.