Investigation of HMG Co A Reductase Inhibitory Activity of Antihyperlipidemic Herbal Drugs Study In Vitro
Hirangi D. Patel, Gaurang B. Shah, Vijaylaxmi Trivedi · 2011
Hyperlipidemia (Elevated cholesterol or triglyceride) is a major cause of atherosclerosis and atherosclerosis associated conditions such as coronary heart disease, ischemic cerebrovascular disease and peripheral vascular disease. Lipid metabolism is regulated by various enzymes. HMG Co A reductase is a key enzyme involving in rate limiting step of cholesterol biosynthesis . Various herbal drugs are potent antihyperlipidemic like Andrographis paniculata, Anthocephalus indicus, Ocimum sanctum, Picrorrhiza kurroa, Plumbago zeylanica, Terminalia arjuna. Their mechanism of action is still unclear. They might be acting through HMG Co A Reductase. Liver microsomal preparation was prepared from high fat diet fed wistar rats. Oxidation of NADPH was measured at 340 nm for HMG Co A reductase activity after addition of 3 different concentration (1 µg/ml, 10 µg/ml, 100 µg/ml) of herbal drugs. Andrographis paniculata (IC -2.959 µg/ml), Anthocephalus indicus (IC -5.310 µg/ml) and Terminalia arjuna (IC -4.526 µg/ml) were found to possess HMG Co A reductase inhibitory activity. From the study it can be concluded that Andrographis paniculata, Anthocephalus indicus and Terminalia arjuna possess HMG Co A reductase inhibitory activity. While Ocimum sanctum, Picrorrhiza kurroa and Plumbago zeylanica do not possess HMG Co A Reductase inhibitory activity. Hyperlipidemia, HMG Co A reductase, NADPH 50