Single point vs. mapping approach for spectral cytopathology (SCP)
Jennifer M. Schubert, Antonella I. Mazur, Benjamin Bird, Milo Miljković, Max Diem · Journal of Biophotonics · 2010
In this paper we describe the advantages of collecting infrared microspectral data in imaging mode opposed to point mode. Imaging data are processed using the PapMap algorithm, which co-adds pixel spectra that have been scrutinized for R-Mie scattering effects as well as other constraints. The signal-to-noise quality of PapMap spectra will be compared to point spectra for oral mucosa cells deposited onto low-e slides. Also the effects of software atmospheric correction will be discussed. Combined with the PapMap algorithm, data collection in imaging mode proves to be a superior method for spectral cytopathology.