Multilayer Microscopic Image Mosaicing Based on 3D Grid Graph
Ligang Miao, Yue Yongjuan, Silong Peng · 2006
This paper presents a novel framework based on three-dimensional (3D) grid graph for automatic mosaic construction of multilayer microscopic images. Firstly the multilayer structure is divided into several slices, and two-dimensional (2D) scanning manner is adopted to sample each slice of the preparation under microscope. Secondly, the overlapping images of same slice are aligned with direct methods, and overlapping images of adjacent slices are aligned with feature-based methods. Thirdly, we use 3D grid graph to represent the neighborhood relations of the microscopic images. Finally, a global image alignment model based on 3D grid graph is proposed to eliminate error accumulation, thus, to get an accurate 3D representation of the multilayer structure