REAL-TIME SPECTROSCOPIC ANALYSIS OF LIGAND-RECEPTOR DYNAMICS
Larry A. Sktar · Annual Review of Biophysics and Biophysical Chemistry · 1987
PERSPECTIVES AND OVERVIEW ....•........••• •.....•••......•• •...... 479 FLOW CYTOMETRY 481 Discrimination of Free and Bound Ligand 481 Calibration and Equilibrium Binding Analysis 483 Kinetic Methods 484 D!sc:im�nation of Internalized Ligand 486 LimitatIOns 488 SPECTROFLUORIMETRIC ANALySIS 488 Discrimination of Free and Bound Ligand 489 Discrimination of Bound and Internalized Ligand 496 LIGAND-RECEPTOR DYNAMICS AND CELL ACTIVATION 497 Fluorescent Ligands 497 Occupancy-Response Relationships 50 I Modeling Ligand-Receptor Dynamics and Its Role in Cell Activation 502