Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Resuscitation Promoting Factor Interacting Protein RipA from M. tuberculosis
Alessia Ruggiero, Flavia Squeglia, Carla Esposito, Daniela Marasco, Emilia Maria Pedone, Carlo Pedone, Rita Berisio · Protein and Peptide Letters · 2009
RipA is an important growth factor of M. tuberculosis. Its depletion produces decreasing bacterial growth and abnormal phenotype. RipA C-terminal fragment (263-472), containing its predicted catalytic domain has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and atomic resolution refinement is in progress. Keywords: Resuscitation promoting factor, crystal, growth factor, x-ray, tuberculosis