Rapid and Inexpensive Method of Diluting Giemsa Stain for Diagnosis of Malaria and Other Infestations by Blood Parasites
Jean-Claude Petithory, F Ardoin, Lawrence R. Ash · Journal of Clinical Microbiology · 2005
For more than a century, Giemsa stain (2) has been used for the staining of blood parasites. The fixation of blood smears in methyl alcohol or the use of the May-Grunwald staining solution (1) is followed by the use of Giemsa stain for 25 to 30 min. Giemsa stain (3 ml) is diluted with buffered distilled water (100 ml) and is the stain of choice for demonstrating the presence of parasites in blood smears.