An interactive image analysis system for mean particle volume estimation using stereological principles
M. C. Moss, Mark A. Oakley Browne, C. V. Howard, David J. Joyner · Journal of Microscopy · 1989
SUMMARY A semi‐automatic image analysis system based on the stereological approach of point‐sampled intercepts for mean volume estimation is described. The method used requires no assumptions about particle geometries and is thus general in its application. To ease implementation in this application, the assumption of particle convexity is made, making the measurement of intercepts easier for the user. The system was tested with computer‐generated sample sections to determine the accuracy and precision of the estimates obtained. The results were found to be within 10% of expected values in all cases. When tested with ‘real’ samples a typical result gave a coefficient of variation of 9% from eight independent estimates of one sample.