Automated reagin test using a particular counter.
Gordon D.W. Curtis, Clive J. Mitchell, H. H. Johnston · Journal of Clinical Pathology · 1977
The automated reagin test (ART) was devised by McGrew et al. (1968) as an adaptation of the rapid plasma reagin (RPR) test (Portnoy et al., 1962) to a continuous flow technique.Results are obtained by the deposition ofcarbon particles onto a continuously moving filter paper strip.We describe a modification which provides an output for a conventional chart recorder from a particle counter. PrincipleAntigen and serum react as in the ART to the point of decantation to the filter paper strip.This decanta- tion together with a second decantation is pulled to waste (Fig. 1).The remaining stream is resampled, diluted, and passed through a flow-cell in the particle counter (Autocounter; Technicon Instruments Co).