The reconstituted (Na,K)-ATPase is electrogenic.
J F Dixon, L E Hokin · Journal of Biological Chemistry · 1980
With the use of the lipid permeant anion ["C]SCN-, which senses potentials across biomembranes, it is shown that addition of MgATP to the outside of (Na,K)-ATPase liposomes, which initiates the pumping of Na' in and K+ out, leads to a MgATP-dependent uptake of [14C]thiocyanate.Based on the steady state ratio of [14C]SCN-(inside:outside), the mean potential was calculated to be 14 mV positive on the inside.Since earlier studies indicated that the K' gradient was steeper than the Na+ gradient (Hilden, S., and Hokin, L. E. (