Nonlinear Fluorescence Spectra Unmixing
Hayato Ikoma, Barmak Heshmat, Gordon Wetzstein, Ramesh Raskar · 2014
We have developed a nonlinear spectral unmixing algorithm that separates fluorescence excitation-emission matrix of multiple fluorophores affected by the inner filter effect. We evaluate this technique on simulated data and demonstrate its superior performance experimentally for a mixture of fluorophores.