Stabilized Self-Sustained Sequence Replication against Evolutionary Instability
Yuji Yamamoto, Shouichi Komiya, Yuzuru Husimi · Chemistry Letters · 2001
Abstract When self-sustained sequence replication, which is an isothermal amplification method of DNA/RNA, was applied to in vitro evolution experiments, it often showed evolutionary instability. A method to prevent this instability was devised using a DNA oligomer of promoter sequence with modified 3'-end.