PVP-augmented LISP and DISP AutoAnalyzer haemagglutination channels.
Joan Crockford, B. P. L. Moore · Journal of Clinical Pathology · 1979
A low-ionic strength-Polybrene (LISP) AutoAnalyzer haemagglutination channel (Lalezari, 1968; Perrault and Hogman, 1971) fails to detect a proportion of antibodies, particularly anti-KI, anti-Lewis, and anti-P1, which can be demonstrated by manual techniques (Moore and Perrrault, 1977).Moore et al. (1977) found that when dialysis was used instead of dilution to achieve a low ionic-strength reaction environment (dialysed serum-Polybrene or DISP), a greater proportion of anti-KI and anti-Lewis antibodies was detected.We have found that the addition of PVP (K-90) to cell suspension medium and Polybrene solution improves the effectiveness of both LISP and DISP channels for antibody detection and identification.Material and methods Figures 1 and 2 are modified from Perrault and Hogman (1971) and Moore et al. (1977).The sampling rate was 60/h with 2:1 sample:wash ratio for both detection and identification of antibodies.REAGENTS Red cell diluent 30 % BSA 20 ml AB serum 15 ml PVP K-90, 50 g/l 8 ml NaCl 9 g/l 35 ml K2EDTA-2H20 1.0 mol/l 2ml (For LISP or DISP, use 9 g/l NaCl in place of PVP) Cell suspension Packed red cells Cell diluent 6 ml 40 ml