A METHOD FOR THE CULTIVATION OF ANAEROBIC SPORE FORMING BACTERIA
A. Kneteman · Journal of Applied Bacteriology · 1957
SUMMARY: A method is described for the cultivation of anaerobes based upon the following two principles: (1) Anaerobiosis is achieved by the addition of an aerobic micrococcus to the nutrient medium itself. After anaerobiosis has been achieved the micrococcus gradually dies, leaving the anaerobes in a pure state. (2) In the plate technique it is necessary to prevent diffusion of oxygen from the air into the medium. This is effected by covering the agar with a film of Saran which is impervious to oxygen. The plate method is carried out in ordinary Petri dishes: anaerobic jars are not used.