Efficient mispriming during apolipoprotein E genotyping
Gabriela Merheb de Azevedo Souza, Nara Lúcia Romano, M.R. Whittle · Jornal Brasileiro de Patologia e Medicina Laboratorial · 2005
The determination of ApoE genotypes is a common clinical laboratory procedure. The most frequently used method involves a PCR amplification of the target region followed by a restriction enzyme digestion of the resultant amplicon to obtain a genotype-specific restriction fragment pattern. We describe an unexpected and surprising difficulty that was encountered during our routine laboratory casework.