Segmentation and analysis of DNA replication sites using deformable models
Raghu P. Menon, Raj S. Acharya, Jagath K. Samarabandu, Ping Chin Cheng, Ronald Berezney · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 1995
Image segmentation is an important aspect of biomedical image analysis. Here we discuss the implementation of a system for segmentation of confocal electron microscope images of DNA within from rat liver cell nuclei. Segmentation is achieved using controlled continuity splines or 'snakes' proposed by Kass, Witkin, and Teraopoulos. A snake is an energy minimizing spline guided by external constraint forces and influenced by image forces that pull it toward features such as lines and edges. They are active contour models. They lock onto nearby edges, localizing them accurately.