Measuring Cell Wall Dimensions using the Distance Transform
G.H.M. van der Heijden · Annals of Botany · 1995
A method has been developed to measure various features of cells and cell walls. It offers possibilities for quantitative analysis of microscopic images which were not feasible before. The method is not based on a model and the only assumption required is that the middle lamella is exactly in the middle of the cell wall of two neighbouring cells. Intercellular spaces are handled correctly with this method. Features can be measured both per individual cell and per frame. It is also possible to measure the variation of the cell wall within a single cell. Hence the variation of the cell wall thickness within cells can be compared to variation between cells. The method is demonstrated for Gerbera but is generally applicable to anatomical studies of plant tissues.Copyright 1995, 1999 Academic Press