A SIMPLE METHOD OF USING GIEMSA'S BLOOD STAIN
Marcus Ward Lyon · JAMA · 1923
The customary method of using Giemsa's stain by employing the freshly diluted stock solution, 1: 10 or 1: 15, with distilled water, makes the use of this excellent stain somewhat troublesome and expensive. The procedure here described simplifies the method, saves stain, and makes it almost as easy to use as is Wright's stain or its modifications. The blood film is fixed by immersing the slide in a jar of methyl alcohol or by pouring the alcohol over it. A half minute appears ample time. After drying, the film side is covered with distilled water, and from three to five drops of the stock Giemsa stain is added to the water in different portions of the slide. A slight rocking of the slide insures uniform mixing of the stain and water. The mixture of the stain and water is allowed to act for about fifteen minutes. The stain and water