A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells
Paul J. Schwartz, Jay A. Blundon, Elizabeth M. Adler · Science s STKE · 2007
This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.