Independent component analysis for three-dimensional optical imaging and localization of a fluorescent contrast agent target embedded in a slab of ex vivo human breast tissue
Mohammad Alrubaiee, Min Qiang Xu, Swapan Kumar Gayen, R. R. Alfano · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 2005
An innovative approach for three-dimensional localization and characterization of a fluorescent target embedded in a turbid medium is presented. The target was a ~4-mm diameter glass sphere with a solution of indocyanine green placed within a 50-mm thick tissuelike phantom with mean free path of ~1-mm at 784-nm and a ~ 26-mm thick ex vivo breast tissue slab. The experimental approach uses a multi-source illumination, and a multi-detector signal acquisition scheme. An analysis scheme based on the independent component analysis from information theory is used for target localization and characterization. Independent component analysis of the perturbation in the spatial intensity distribution of the fluorescent signal measured on the exit plane of the turbid medium locates the embedded objects. The location and size, of the embedded objects are obtained from a Green's function analysis and back-projection Fourier transform of the retrieved independent components.