Optimizing Kinase Assays for Ultrahigh-Throughput Profiling using the Kinase-Glo plus Assay
Tracy J Worzella, Aoife Gallagher · JALA Journal of the Association for Laboratory Automation · 2007
Here we demonstrate the concept of kinase profiling using a panel of four kinases and 10 kinase inhibitors. We used the Kinase-Glo Plus Luminescent Kinase Assay and the Deerac Fluidics Equator HTS noncontact liquid-handling system to design and perform an ultrahigh-throughput profiling screen. We present IC50 values for each test compound and show how such profiling can streamline the initial drug discovery process.