Automatic segmentation of cells from microscopic imagery using ellipse detection
Nawwaf Kharma, Hussein Moghnieh, Jie Yao, Yana Guo, Ayman AbuBaker, Janet Laganière, Guy Armand Rouleau, Mohamed Cheriet · IET Image Processing · 2007
Cell image segmentation is a necessary first step of many automated biomedical image-processing procedures. There certainly has been much research in the area. To this, a new method has been added, which automatically extracts cells from microscopic imagery, and does so in two phases. Phase 1 uses iterated thresholding to identify and mark foreground objects or ‘blobs’ with an overall accuracy of >97%. Phase 2 of the method uses a novel genetic algorithms-based ellipse detection algorithm to identify cells, quickly and reliably. The mechanism, as a whole, has an accuracy rate >96% and takes <1 min (given our specific hardware configuration) to operate on a microscopic image.