On the New Graphical Expression of Plasma Protein Fraction
Hideyuki Ariga, 貞彦 赤井, Hiroshi Fukai · SEIBUTSU BUTSURI KAGAKU · 1953
Results, which are obtained by electrophoretical method, have too many factors to be expressed in a simple manner. At least, three factors must be considered in the explanation of electrophoretic results; they are: 1) there are four or five components to express serum protein fractions (Alb., α-Glob., β-Glob. and γ-Glob) or plasma fractions (serum components plus fibrinogen), 2) electrophoretic pattern must be disscussed from vorious points of view, nomely, an alternation in forms and mobilities from the regular plasma or serum components, or an appearances of unusual component, 3) it is difficult to comprehend the increase or decrease by sinply observing the numerical changes of protein fractions.Agogram and Serogram, designed by K. Honda et al and H. Kanagami et al, are new excellent graphical expressions of electrophoretical serum protein components, taking into account factors 1) and 3) mentioned above. We disscussed Hond's Agogram and revised it and designed “Agogram”.There are some differencess between these two expressions. a) “Agogram” uses percentage to express the electrophoretic values. Because, we can obtain only percentage of protein fraction directly from the pattern, but not each protein concentration (g/dl) as applied in Agogram. And it is one of the charactsristics of the electrophoresis that we can catch correlational ratios. of protein components. Moreover, it is liable to produce errors to convert percentage of fraction into concentration values of each components. b) Agogram express the values of increase or decrease of components. But normal protein fraction has each different level, and for example, when both φ and γ-Glob. increased 10%, Agogram will express the increase of both components in the same degree, in spite of the fact that percentagec in increase in φ-component is much larger than that in γ-Glob. Accordingly our “Agogram” expresses the ratio of increase or decrease calculated by the following formula:A-B/A×100=Ratio of increase or decreaseA: value of normal subjects (%)B: value of the materialWe applied this “Agogram” in clinical use together with the electrophoretic pattern and the values of each components, and could make easier and clearer interpretation of the protein fluctuations of pre-and post-operative period, carcinomas and various liver diseases.