Comparative study between BD Falcon® and Corning® cell culture flask on viability and growth of breast, colon, liver and lung cancer cell lines
M. A. Nurul Afiqah, Z. Izmil Haikal, E. Zolkapli, W. I. Wan Iryani · 2012
Cancer is one of the greatest threats in the world affecting millions of people worldwide. In order to treat the disease, several methods have been designed including the use of cancer cell line research model in developing the most effective drugs. However, external environment of cancer cell lines e.g. substance of flask's surface may affect the investigation. Subsequently, the results are probably not valid for further study i.e. in giving positive false results. Therefore, in this study, human colonic (HCT116), human breast (MCF7), human lung (A549) and human liver (HepG2) cancer cell lines were cultured in two most common flasks i.e. Corning Costar ® and BD FalconTM. Viability and growth of the cells was observed using trypan blue staining and an inverted microscope. There is no significant difference in viability and growth of cancer cell lines which were cultured in different flasks. It suggests that cancer cell lines are robust and can grow in both cell culture flasks successfully.