Computer‐assisted three‐dimensional reconstruction technology in plant cell image analysis: applications of interactive computer graphics
M. Tuohy, Cameron A. McConchie, R. Bruce Knox, LM Szarski, Adam P. Arkin · Journal of Microscopy · 1987
SUMMARY Sequences of more than 100 serial thin sections were cut of entire generative cells of pollen of Brassica campestris. Using transmission electron micrographs, outlines of the cell nucleus, vacuoles and mitochondria were digitized and stored on disc using the Carl Zeiss‐Kontron Videoplan Image Analysis System. The data collected concerned the geometry of cuts and logical collection of cuts into structures, both internal and external. A data translation and surface generation system was established to transfer these data to a VAX‐751 computer to establish an interface to the Intergraph's Interactive Graphics Design System (IGDS). Definition of the surfaces is based on the translated boundaries and structural configuration, enabling three‐dimensional visualization of: (a) the wireframe model; (b) the overall model with removed hidden lines, including colour or monochrome shading; and (c) the shaded image of the internal structures with superimposed wireframe representation of the external structures.