A Manual of Morphometry in Diagnostic Pathology
Gerard Slavin · Journal of Clinical Pathology · 1984
Letters to the Editor for enzyme histochemistry is easily adapted to immunohistochemistry.With TNBT a distinct black precipitate was obtained, whereas NBT did not result in an acceptable product.TNBT staining was somewhat less distinct than azo dye coupling reactions.With both newly introduced methods plasma cells and extravasal deposited immunoglobulins were easily demonstrated in human synovial tissue.When the sensitivity of the standard methods for alkaline phosphatase were compared with either the new azo coupling method with variamine blue salt or the McGadey technique, no differences in the sensitivity could be seen (Table ).But the four methods demonstrating alkaline phosphatase labelled antibodies were less sensitive than the PAP technique in identifying endogenous IgG within conventionally processed tissue sections of human synovium.