Methodology for straining engineered vocal fold tissues at audio frequencies
Sarah A. Klemuk, Ingo R. Titze, Steven G. Gray · The Journal of the Acoustical Society of America · 2001
Engineered vocal fold tissues will be grown under conditions of shear strain, the magnitude of which is determined by known strains during human vocal fold oscillation. A cup and plate system is used with the Bohlin CVO 120 rheometer to apply a shear strain of approximately 0.4 radians at frequencies of 20–60 Hz. Baseline viscoelastic measurements are recorded for the synthetic scaffolding material into which different cell lines will be injected and grown to form their own matrix. The methodology allows duration and repetitions of strain exposure to seeded scaffolding to be varied and for subsequent viscoelastic measurements to be compared. [Work supported by NIDCD.]