Base-calling for Illumina's next-generation DNA sequencing systems via Viterbi algorithm
Shreepriya Das, Haris Vikalo · 2011
We look at statistical methods to reduce error rates for II lumina's next-generation sequencing platforms. In particular, we propose a computationally tractable parametric model for Illumina's Genome Analyzer which is amenable to the use of dynamic programming ideas. We investigate the performance of the proposed algorithm for base-calling in terms of error rates, compare it with the state-of-the-art basecalling schemes for this platform, and demonstrate its feasibility on experimentally obtained data.