Localization and cross section reconstruction of fluorescent targets in ex vivo breast tissue using independent component analysis
Mohammad Alrubaiee, Min Qiang Xu, Swapan Kumar Gayen, R. R. Alfano · Applied Physics Letters · 2006
An information theory based approach is introduced for the detection and three-dimensional localization of fluorescent targets embedded in a turbid medium. The approach uses multisource illumination of the medium, multidetector transillumination acquisition of fluorescence signal, independent component analysis for target detection and localization, and a back-projection algorithm for reconstruction of target cross section. The efficacy of the approach is demonstrated by locating and estimating the cross section of a fluorescent target embedded in a 100×100×26mm3 ex vivo human breast tissue specimen and in a tissue-simulating turbid medium of thickness 50 times the transport mean free path.