In Vitro Methods for Detecting Cytotoxicity
Marion F. Ehrich, Lioudmilla Sharova · Current Protocols in Toxicology · 2000
Toxicant-induced damage to cells (cytotoxicity) can depress cell growth, compromise intracellular metabolic processes, and/or cause loss of cell viability. Methods that indicate these cytotoxic changes following toxicant exposures are provided, including [³H]thymidine uptake to measure cell growth, MTT dye conversion to detect changes in metabolic activity, and trypan blue uptake to indicate loss of cell viability.