Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility-0
Sourabh Kharait, Sampsa Hautaniemi, Shan Wu, Akihiro Iwabu, Douglas A. Lauffenburger, Alan Wells · DSpace@MIT (Massachusetts Institute of Technology) · 2011
Copyright information:Taken from "Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility"BMC Systems Biology 2007;1():9-9.Published online 29 Jan 2007PMCID:PMC1839898.ere grown on these surfaces for 24 hours in complete growth medium and quiesced for another 24 hours in medium containing 0.5% dialyzed FBS. EGF was added for a period of 1 hour, cells washed once with PBS and lysed. Cell lysates were resolved using SDS-PAGE and immunoblotted using specific antibodies for various phosphorylated proteins. At least 5 replicates for each signaling protein were created for polynomial modeling. Actin served as a loading control.