HiCT: High Throughput Protocols For CPE Cloning And Transformation
Ralf Beer, Tania Christiansen, Konrad Herbst, Nikolaos Ignatiadis, Ilia Kats, Nils Kurzawa, Johanna Meichsner, Sophie Rabe, Anja Riedel, Joshua Sachs, Julia Patricia Schessner, Florian Schmidt, Philipp Walch, Lorenz Adlung, Katharina Genereith, Fanny Georgi, Tim Heinemann, Hannah V. Meyer, Dominik Niopek, Barbara Di Ventura · DSpace@MIT (Massachusetts Institute of Technology) · 2013
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High Throughput Circular Extension Cloning and Transformation (HiCT). Description of construct libraries generated by the HiCT method can be found at http://2013.igem.org/Team:Heidelberg/Indigoidine. This RFC also points out further optimization strategies with regard to construct stability, reduction of transformation background and the generation of competent cells.