A high-throughput screening method for general cytotoxicity part I Chemical toxicity
Amílcar Roberto, Paulo Pliças Caetano · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · 2005
A high-throughput assay intended for screening and ranking the general toxicity of physical or chemical xenobiotics based on a Saccharomyces cerevisiae model was developed. In order to standardise the running conditions for the technique the relationship between the absorbance at 525 nm and the number of cells/ml suspended in YPD culture medium (YPD - yeast extract 1%, peptone 0.5% and glucose 2%) was determined and a standard curve, described by the equation Y=6.8219E-08X + 0.0327. Cultures of yeast in YPD medium were exposed to different concentrations of nicotine and the growth inhibition evaluated. We could demonstrate by covariance analysis the reproducibility of the results obtained and that method is valid and suitable for its aims.